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JWH 250 binds with higher affinity and acts as an agonist at G protein-coupled cannabinoid receptors CB1 and CB2. The strategy of analysis for JWH 250 consists of using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). This analytical system has long been accustomed to measure overall concentrations in wastewater samples, human serum, and urine samples from topics who may have smoked cannabis.

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In addition,The medication is often a phenylacetylindole and it goes about being an agonist at CB1 and CB2 receptors. It's a partiality of ~eleven nM at CB1 and ~33 nM at CB2.

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